MICRODETERMINATION OF PURINES AND PYRIMIDINES IN BIOLOGICAL MATERIALS

Polarography of biological purines and pyrimidines.

Since electron-transfer reactions are so important in biological systems, information which can be obtained in respect to the energy levels (electrical potentials) and reaction pathways involved should be helpful in the understanding, control and utilization of such reactions. Polarographically determinded half-wave potentials ( and electrode reaction paths or mechanisms readily provide such da...

Nucleotide Metabolism – Purines and Pyrimidines

Nucleotide metabolism results in the synthesis of the four nucleotides that form DNA. These are further divided in two categories: purines and pyrimidines. Their construction and degradation takes place in a progression of single steps, while different enzymes and cofactors are involved in the process. Beside degradation, there is also a form of reprocessing purine and pyrimidine nucleotides, d...

Receptors for purines and pyrimidines.

Microdetermination of calcium and magnesium in biological materials.

The use of the dye calcon (1-(2 hydroxy-1-naphthylazo)-2-naphthol-4 sulphonic acid) for the estimation of calcium using E.D.T.A. and a commercial photoelectric titrimeter is described. The interfering effects of magnesium and phosphate have been overcome. The method has been extended to estimations on biological materials. Results on 55 sera show that the E.D.T.A./calcon method gave slightly lo...

Nucleotide Metabolism – Purines and Pyrimidines

Nucleotide metabolism results in the synthesis of the four nucleotides that form DNA. These are further divided in two categories: purines and pyrimidines. Their construction and degradation takes place in a progression of single steps, while different enzymes and cofactors are involved in the process. Beside degradation, there is also a form of reprocessing purine and pyrimidine nucleotides, d...